Objective The clinical significance of plasma CD89, PRDX1 and AOPP in the patients with non-renal henoch-schonlein purpura (HSP) and renal purpura was analyzed retrospectively, in order to provide references for the pathogenesis of HSP and early identification of renal purpura. Methods One hundred and four patients diagnosed with HSP in the Affiliated Hospital of Chengde Medical University from December 2020 to April 2023 were chosen as the study object. Patients were divided into non-renal purpura group (47 cases) and renal purpura group (57 cases) according to the results of urine test. Fifty-seven healthy people at the same time were chosen from the physical examination department as normal control group. The plasma levels of CD89, PRDX1 and AOPP in each group were measured by ELISA. Kruskal Wallis test and binary logistic regression were used for data comparison analysis and correlation analysis. ROC curve was applied to analyze the value of plasma CD89, PRDX1 and AOPP in the diagnosis of renal purpura. Results The plasma levels of CD89, PRDX1 and AOPP of renal purpura group were significantly higher than the levels of normal control group and non-renal purpura group, the results of non-renal purpura group were significantly higher than those of normal control group, there were statistically significant differences (P<0.05). Multivariate Logistic regression equation indicated that the effect of them on renal purpura was statistically significant. ROC curve shows that the combination of three factors has higher value in predicting the occurrence renal purpura. Conclusion Plasma CD89, PRDX1 and AOPP may be involved in the pathogenesis of HSP and renal purpura. The combination of plasma CD89, PRDX1 and AOPP has good clinical value in the diagnosis of renal purpura .

Objective To study the CD68 and VEGF expression in skin lesion tissue and their functions in pathogenesis of the erythema nodosum. Methods The expression of CD68 and VEGF in the skin lesions of 49 cases of erythema nodosum patients (disease group) and 10 cases of normal human (control group) were detected by MaxVisionTM immunohistochemical staining method. Results Compared with the normal control group(60.00%), the positive expression rate of CD68 in disease group(95.92%) was increased significantly, with statistical differences between two groups(P<0.01). Compared with the normal control group(60.00%), the positive expression rate of VEGF in disease group(95.92%) was increased significantly, with statistical differences between two groups (P<0.01). Conclusion Higher expression of CD68 and VEGF in skin lesions was concluded that active inflammatory cells and injury of endothelial cell were participated in pathogenesis of erythema nodosum.

Objective In order to investigate the application of anti-Dsg1and anti-Dsg3 antibody in diagnosis and treatment of patients, the level and changes of anti-Dsg1and anti-Dsg3 antibody in 18 patients with pemphigus and the relationship between the antibody index and the severity of the disease were analyzed. Methods 18 patients were diagnosed for pemphigus by direct immunofluorescence and pathology, and the patient's pathogenetic condition was assessed by ABSIS standard.The level of Dsg1 and Dsg3 of patients and nomals were detected by enzyme-linked immunosorbent assay(ELISA). Statistics was performed to analyze the relationship and significance of each index at last. Results Among the 18 patients, 13 were Dsg1 positive and 10 Dsg3 positive . The index of Dsg1 and Dsg3 after treatment were decreased than before, and the difference was statistically significant (P<0.05). Of the 20 nomals, 2 were Dsg1 positive and 1 was Dsg3 positive. The sensitivity of Dsg1 and Dsg3 antibody detection was 72.22% and 55.56% respectively, and the specificity was 90% and 95% respectively. The index of Dsg antibody was correlated with the ABSIS score (ALL P<0.05). Conclusion The Dsg antibody level of patients with pemphigus was correlated with the severity of the disease with high sensitivity and specificity. The detection of Dsg antibody level can be used for diagnosis and guidance of state and treatment of the disease.

Objective To study the features of clinical, dermoscopic and pathological of Seborrheic Keratosis and analyze their correlation. Methods Sixty-one patients with Seborrheic Keratosis diagnosed by histomathological examination and dermoscopy, who were admitted to the Affiliated Hospital of Chengde Medical University from March 2017 to December 2019, were selected to analyze the relationship between different clinical features, dermoscopy findings and pathological features of skin lesions. Results Under the dermoscope of fissures/ brain pattern appearance, comedo-like openings,milia-like cysts, the proportion of clinical papule, plaque and nodule was higher than that of macule, patch and verruca/papilla, and the differences were statistically significant (P<0.05). There were positive correlation between fissures/brain pattern appearance and hyperkeratosis and acanthosis (P<0.05). There were positive correlations between comedo-like openings, punctiform/hairpin blood vessels, milia-like cysts and acanthosis (P<0.05). Conclusion The diagnostic rate of seborrheic keratosis could be improved by the combination of dermoscopic examination and clinical features, and the accurate rate of diagnose of dermoscopy and pathology is highly consistent.