Objective: To characterize the clinical distribution and drug resistance of hospital-acquired infections due to Enterococcus spp and provide basis for clinical treatment. Methods: The clinical data of hospital-acquired enterococcus infection patients from 2013.1 to 2015.12 in Chinese PLA General Hospital were retrospectively analyzed. Results: A total of 883 strains of Enterococci were isolated in this study. Most of them were isolated from ICU (292 strains, 33.07%) and hepatobiliary surgery department (125 strains, 14.16%); Urine was the most common sources of these strains (347, 39.30%), followed by body fluids (315, 35.67%) and blood samples (202, 22.88%); The top organism was Enterococcus faecium (596, 67.50%), followed by Enterococcus faecalis (244, 27.63%). The resistance rates of Enterococcus faecium to ampicillin, penicillin, streptomycin, tetracycline, erythromycin, vancomycin and ciprofloxacin were obviously higher than Enterococcus faecalis (P<0.05). Conclusions: Enterococcus faecium and Enterococcus faecalis are the main pathogens in hospital-acquired enterococcus infection, and Enterococcus faecalis has higher resistance rate. It's necessary to choose the appropriate antibiotics according to drug susceptibility test in clinical treatment.

Objective: To explore the effects of aminophylline on proliferation of adipose tissue-derived stem cells (ASCs) of SD rats. Methods: ASCs of SD rats were isolated and cultured to the third generation. Trypan blue staining was used to detect the survival rate of ASCs, MTT methods to draw growth curve after the ASCs were cultured with aminophylline of different concentration (0μg/ml, 5μg/ml, 10μg/ml, 20μg/ml) for 48h. Results: There was no significant difference about survival rate of ASCs in different concentration aminophylline group (P>0.05). There was no significant difference about proliferation of ASCs at same culture time and of different concentration of aminophylline (P>0.05). Conclusions: Treatment concentration of aminophylline has no obvious effects on survival rate and proliferation of ASCs in vitro.