Objective To investigate the impact of YTH N6 methyladenosine RNA binding protein 3 (YTHDF3) on the proliferation, migration, and apoptosis of human colorectal cancer cell line HCT-116. Methods The cells were divided into a negative control group, interference groups 1, 2, and 3. Small interfering RNA targeting YTHDF3 was transfected into colorectal cancer cells HCT-116. The expression of YTHDF3 in each group was assessed using qRT-PCR and Western blot analysis. Cell proliferation was evaluated using CCK-8 assay and plate cloning assay, while cell migration was assessed using transwell assay and scratch assay. Flow cytometry was used to measure cell apoptosis. Results The silencing efficiency of HCT-116 cells was validated through qRT-PCR and Western blot analysis. Transfection of small interfering YTHDF3 effectively reduced the expression of YTHDF3 in HCT-116 cells compared to the negative control group. Silencing YTHDF3 can significantly inhibited cell proliferation and migration while promoting apoptosis in HCT-116 cells. Conclusion Silencing YTHDF3 can decelerates cell proliferation and migration while enhancing apoptosis in HCT-116 cells.

Objective To investigate the expression of IFITM1 in colon cancer and the mechanism of its promotion of colon cancer cell proliferation. Methods The expression and prognosis of IFITM1 in colorectal cancer were analyzed by bioinformatics, and the correlation between IFITM1 and β-catenin was researched. Immunohistochemical SP staining was examined to observe the expression of IFITM1 and β-catenin in colon cancer tissues. The overexpressed cell model of IFITM1 was constructed, the changes of cell proliferation capacity were detected by CCK-8 assay, and the changes of TCF/LEF transcription factor activity were tested by dual luciferase reporter gene. The results of dual luciferase reporter gene showed that the promoter activity of OE-IFITM1 group was higher than that of OE-NC group (P<0.05), and there was no difference between OE-NC group and MOCK group (P>0.05). The expressions of key proteins β-catenin, C-myc and CyclinD1 in the Wnt/β-catenin signaling pathway were detected by Western blot. Results The results of online database analysis showed that IFITM1 was highly expressed in colon cancer (P<0.05), and significantly correlated with poor prognosis. There was positive correlation between IFITM1 and β-catenin. Immunohistochemical results showed that the expression level of IFITM1 was correlated with tumor diameter and tissue invasion degree (P<0.05), ranther than others factors(P>0.05). The expression of β-catenin was only correlated with tumor diameter(P<0.05), instead of other factors(P>0.05), and there was a positive correlation between IFITM1 and β-catenin. After overexpression of IFITM1 in HCT-15 colon cancer cells, the proliferation capacity of OE-IFITM1 group was significantly higher than that of OE-NC group (P<0.05), but there was no difference between OE-NC group and MOCK group (P>0.05). Western blot results showed that the expression levels of β-catenin, CyclinD1 and C-myc in OE-IFITM1 group were higher than those in OE-NC group (P<0.05), but there was no difference in protein expression between OE-NC group and MOCK group (P>0.05). Conclusion The expression of IFITM1 in colon cancer tissues is significantly higher than that in para-cancer tissues, and IFITM1 may promote cell proliferation through Wnt/β-catenin signaling pathway.