Objective To investigate the expression of androgen receptor (AR) and Ki-67 in triple-negative breast cancer, and to study their relationship with patients' prognosis. Methods Eighty-eight female TNBC patients treated at Chaoyang Central Hospital from January 2015 to May 2020 were included in the observation group, while 89 female NTNBC patients were included as a control group. We examined the expression of AR and Ki-67 and observed the differences in their expression in triple-negative breast cancer, and analysed their relationship with patients' prognosis. Results The expression rate of AR in the observation group was lower than that in the control group (P<0.05), while the expression rate of Ki-67 in the observation group was higher than that in the control group (P<0.05). The expression of AR and Ki-67 is related to tumor diameter and the number of lymph node metastases (P<0.05). COX regression analysis showed that pathological grade, number of lymph node metastases, AR and Ki-67 expression were independent influences on the prognosis of triple-negative breast cancer (P<0.05). Conclusion AR and Ki-67 were closely related to the prognosis of triple-negative breast cancer. Patients with positive AR expression and low Ki-67 expression suggested a better prognosis and longer survival time.