Objective To investigate the effect of knocking down LncRNA TCONS-00022381 on apoptosis of human gastric cancer cells MKN45. Methods Culturing Human gastric cancer MKN45 cells and dividing them into three groups. Cells in each group were transfected using the Liposome transfection method, the siRNA targrting LncRNA TCONS-00022381 was transfected into the si-TCONS group, the negative siRNA was transfected into the NC group, and the Blank group was cultured in routine. The cell viability of each group was measured after 24h, 48h, and 72h of transfection using the CCK8 assay TCONS knockdown efficiency was detected by RTqPCR after 24h of transfection. The expression of Bcl-2 and Bax was detected by western blotting after 48h hours of transfection. The apoptotic rate of cells was detected by flow cytometry after 48h of transfection. Results The siRNA targeting LncRNA TCONS-00022381 significantly inhibited its expression in MKN45 cells (P<0.001). Compared with the Blank group and the NC group, the viability of MKN45 cell in the si-TCONS group decreased significantly (P<0.001). The expression of Bcl-2 was significantly down-regulated (P<0.01). The expression of Bax was significantly up-regulated (P<0.01). The rate of cell apoptosis increased significantly (P<0.05). Conclusion Knock down of LncRNA TCONS-00022381 may promote the apoptosis of MKN45 cells by regulating the expression of Bcl-2 and Bax.

Objective To explore the expression relationship and significance of intramedullary IL-4 and CD34 in rabbit VX2 bone tumors. Methods Thirty Japanese white rabbits were divided into experimental group and control group with 15 rabbits each by random number method. In the experimental group, the left tibia was implanted with VX2 tumor tissue through the tibial plateau to make a bone tumor model. The control group adopted the same operation method but did not implant the VX2 tumor tissue. Closed biopsy was used to extract the bone marrow of the left proximal tibia 7 days and 14 days after operation, and the levels of IL-4 and CD34 in all specimens were detected by enzyme-linked immunosorbent assay (Elisa). At the same time, 14 days after operation, tibia tissue was taken for histopathological examination after execution. Results 30 rabbits were successfully modeled and included in the study. On the 7th day after operation, the intramedullary IL-4 and CD34 contents of rabbits were significantly higher than those before operation, and the difference was statistically significant (P<0.05). On the 14th day after the operation, the IL-4 and CD34 levels were significantly higher than those on the 7th day after the operation. The difference was statistically significant (P<0.05). The pathology showed that the tumor tissue was in the tibial marrow cavity in the bone tumor model group 14 days after the tumor was implanted. Significant tumour growth with tumour cells visible in the bone cortex. Conclusion Within 14 days of modeling rabbit VX2 bone tumor animal model, as the tumor proliferates rapidly, the body's anti-tumor immune function is gradually inhibited, which is manifested by the increased expression of IL-4 and CD34.