Objective To observe the clinical effect of minimally invasive holmium laser bladder tumor resection for superficial bladder tumors through the urethra. Methods From June 2016 to December 2019, 64 patients with superficial bladder tumor who admitted to our hospital were selected as the research object, and divided into HOLRBT group and TURBT group (32 cases each) by random double blind method. The group was treated with transurethral holmium laser bladder tumor resection, and the TURBT group was treated with transurethral resection of bladder tumor. Observation of the operation, stress response and complications of the two groups was performed. Results Compared with the TURBT group, the intraoperative blood loss, bladder irrigation time, urinary indwelling time and hospital stay in the HOLRBT group were shorter (P<0.05), but the operation time of two groups was not statistically significant (P>0.05). The stress response indexes (E, ATⅡ, NE) in the HOLRBT group during operation and 6 hours after operation were significantly lower than those in the TURBT group (P<0.05). The total postoperative complication rate (9.38%) in the HOLRBT group was significantly lower than TURBT Group (31.25%) (P<0.05). Conclusion Transurethral minimally invasive holmium laser bladder tumor resection can reduce the amount of intraoperative bleeding, shorten the bladder irrigation time, indwelling catheter time and hospitalization stay, improve its stress response level, and reduce the incidence of postoperative complications, which is worthy of extensive clinical promotion.