Objective To analyze the effect and safety of phloroglucinol on the observation of the esophagogastric junction during painless gastroscopy. Method A control group consisting of 135 cases underwent painless gastroscopy, and an observation group consisting of 115 cases(painless gastroscopy plus phloroglucinol) was randomly formed from patients who underwent painless gastroscopy between September 2022 and February 2023 based on the odd-evenness of their registration numbers. The esophagogastric junction observation effect, lesion detection rate, endoscopic features of lesions, pathological outcomes, and intraoperative and postoperative adverse responses of the two patient groups were noted and monitored. Result This portion of the observation group had a greater observation rate of the esophagogastric junction and a higher rate of lesion detection than the control group, and the difference was statistically significant (P=0.038, P=0.035). In comparison to the control group, the observation group had a greater detection rate of non-red lesions; this difference is statistically significant (P=0.003). Between the two groups, there was no statistically significant difference in the occurrence of adverse responses or pathological results (P>0.05). The difference was statistically significant (P=0.003). The incidence of adverse responses and abnormal outcomes did not change statistically significantly between the two groups (P>0.05). Conclusion The esophagogastric junction's observation impact can be enhanced by applying phloroglucinol before the painless gastroscopy, which will increase the likelihood of lesions being discovered. Finding easily overlooked non-red lesions is one of its specific uses. Additionally, it is more secure and has fewer adverse effects.

Objective To investigate the effect of skeletal muscle contusion on iron metabolism and its correlation with ferroptosis in rats. Methods Male SD rats were randomly divided into control group and blunt contusion model group. The model group was selected at 1d, 3d, 5d, 7d and 14d after injury, respectively. HE staining was used to observe the pathological changes of the injured tissue, and Prussian blue staining was used to observe the iron ion deposition in the injured area. ELISA method was used to detect the changes of serum ferritin, muscle tissue reactive oxygen species, α-ketoglutarate and heat shock protein 1. Results HE staining showed the rupture of muscle membrane, nucleolysis and inflammatory cell infiltration in the contusion area, which began to decrease after 5 days. The number of new skeletal muscle cells in the contusion area increased further on 14 days, and the number of intercellular collagen fibers increased. Prussian blue staining showed that iron deposition increased gradually after contusion, the highest in fifth day group, and then decreased. ELISA results showed that serum ferritin, ROS and α-KG in the model group increased gradually after injury, and the difference was statistically significant compared with the control group (P<0.01). HSPB1 first increased and then decreased after injury, and the difference was statistically significant compared with the control group (P<0.01). Conclusion Abnormal iron metabolism is closely related to ferroptosisafter skeletal muscle contusion, and cell ferroptosis also occurs after skeletal muscle acute injury.

Objective The distribution and length of the tear area and the degree of the contracture of the broken end of the rotator cuff tear image were measured and compared with the tear morphology seen during the operation. Methods From October 2019 to December 2021, a total of 38 patients who met the research criteria and underwent arthroscopic or open repair due to rotator cuff tear were collected. The distribution area, length, and degree of contracture of rotator cuff tear were measured by this method, and the general shape of the tear was described before surgery and compared with the intraoperative situation. The main comparison parameters included distribution area and length of preoperative MRI oblique sagittal and intraoperative tears, and degree of oblique coronal contracture. Paired sample rank sum test was used for statistical analysis. The preoperative MRI measurements and intraoperative measurements were compared for statistical differences. At the same time, the common area of tears was calculated, and the consistency of preoperative and intraoperative tear morphology was compared. Results A total of 38 patients (20 males and 18 females), ranging in age from 37 to 73 years, were enrolled. There was no significant difference in rotator cuff tear length and contracture degree measured by oblique coronal MRI before operation (P=0.427; P=1.000). There was no significant difference in the length of rotator cuff tear and the degree of contracture between the preoperative MRI and the actual incision measurement (P=0.341; P=1.000). There was no significant difference between preoperative MRI and actual intraoperative microscopic measurement (P=0.865; P =1.000). Statistical analysis of rotator cuff tear zones showed that there was no significant statistical difference between the origin and end areas of the tear shown by preoperative MRI and the actual intraoperative tear origin and end areas (P=0.180; P=0.411), and the distribution frequency of tear zone was high in zones 1~5. Conclusion Preoperative MRI measurement and description of tear partition can show the tear morphology relatively intuitively, and has good consistency with the tear morphology seen under microscope and incision. It is helpful to plan the distribution of anchor and suture method during operation. Shorten the learning curve of shoulder arthroscopy. To improve the consistency of interpretation of tear morphology between surgeons and radiologists.

Objective To investigate the expression characteristics and clinical significance of P-selectin in benign and malignant hydatidiform mole tissues. Methods 50 cases of benign moles and 22 cases of malignant moles were selected. qRT-PCR technology is used to detect the expression level of P-selectin mRNA. Immunohistochemistry SP method and Western Blot technology comprehensively detected the expression level of P-Selectin protein. Results Compared with benign hydatidiform mole tissue, the relative expression of mRNA and protein and protein positive expression rate of P-selectin in malignant hydatidiform mole tissue were significantly higher (P<0.05). Conclusion P-selectin participates in the development of malignant hydatidiform mole at the gene and protein level, and is expected to become a diagnostic indicator for evaluating benign and malignant hydatidiform moles, providing new ideas for clinical targeted therapy.

Objective To check the differential expression of apoptosis-related factor Bid in adenocarcinoma of stomach tissue an and analyze its clinical significance. Methods 60 cases of adenocarcinoma of stomach tissues were the experimental group, and 55 cases of normal gastric mucosal epithelium tissue were the control group. The expression of Bid protein and mRNA was comprehensively detected by combined immunohistochemical SP method, Western blot and qRT-PCR technology. Results Compared with normal gastric mucosal epithelium tissue, the positive expression level of Bid protein and the relative expression level of mRNA in adenocarcinoma of stomach tissue are reduced, and the differences are statistically significant (P<0.05). In protein level and gene level, the Bid molecule expression level in adenocarcinoma of stomach tissues was related to the degree of differentiation, lymph node metastasis, clinical TNM stage and infiltration depth(P<0.05). Conclusion The apoptosis-related factor Bid plays a vital role in the occurrence and development of adenocarcinoma of stomach, and it may become a new target for the treatment of adenocarcinoma of stomach.

Objective To comparatively analyze the effects of laparoscopic and open left hepatectomy on intrahepatic bile duct stones. Methods Ninety-one patients with intrahepatic bile duct stones were divided into observation group (48 cases) and control group (43 cases) according to different surgical methods. The patients in observation group underwent laparoscopic left hepatectomy and the patients in control group underwent traditional open left hepatectomy. The intraoperative conditions (operation time, intraoperative blood loss), postoperative conditions (postoperative exhaust time, postoperative time of leaving bed, postoperative hospitalization time), postoperative complications (pneumonia, incision infection, bile leakage, abdominal bleeding), residual stones and recurrence of stones of two groups were compared. Results The operation time of patients in observation group was significantly longer than control group (P<0.05); While the postoperative exhaust time, postoperative time of leaving bed and postoperative hospital stay of were significantly shorter (P<0.05). There was no significant difference in intraoperative blood loss, the incidence of postoperative complications, the residual stone rate, and the recurrence rate between two groups (P>0.05). Conclusion Treating intrahepatic bile duct stones with laparoscopic left hepatectomy has the advantages of less trauma and rapid postoperative recovery, and is worthy of popularization and application in hospitals with conditions.

Objective: To investigate the preventive effects of rectal administration of indomethacin on pancreatitis and hyperamylasemia after endoscopic retrograde cholangiopancreatography (ERCP) in elderly patients with choledocholithiasis. Methods: 70 elderly patients with choledocholithiasis undergoing ERCP were randomly divided into indomethacin group (35 cases) and placebo group (35 cases). The patients in indomethacin group were given indomethacin through rectum 30 minutes before ERCP, the patients were given placebo through rectum. The serum amylase level of patients before ERCP, 3h and 24h after ERCP were detected; and to determine whether patients with pancreatitis and hyperamylasemia. Results: The serum amylase level of patients in indomethacin group 3h and 24h after ERCP were obviously lower than placebo group (P<0.05). The incidence rate of pancreatitis and hyperamylasemia after ERCP of patients in indomethacin group were respectively 5.71% (2/35) and 17.14% (9/35), which were all obviously lower than placebo group (P<0.05). Conclusions: Rectal administration of indomethacin before ERCP can remarkably reduce the incidence of pancreatitis and hyperamylasemia, so it is worth of clinical promotion.