Objective To explore the expression and biological behavior of Ribonucleotide Reductase Regulatory Subunit M2 (RRM2) in Non-Small Cell Lung Carcinoma (NSCLC). Methods Gene expression profiles and clinical data of NSCLC patients were extracted from The Cancer Genome Atlas (TCGA) database. The expression of RRM2 gene in normal tissues and NSCLC tissues was analyzed, and survival curves werewere plotted by utilizing the clinical data of the NSCLC patients. Quantitative Real-Time PCR (qPCR) was performed to determine RRM2 expression levels and verify silencing and overexpression efficiency in cell lines. Western Blot was applied to detect the protein expression of the RRM2 gene in cell lines and to validate the efficiency of its silencing and overexpression in cell lines. Cell Counting Kit-8 (CCK-8), flow cytometry and Transwell assays were conducted to detect cellproliferation, apoptosis, invasion, and migration abilities. Results RRM2 expression was significantly elevated in Lung Squamous Cell Carcinoma (LUSC) and Lung Adenocarcinoma (LUAD) tissues compared to normal controls (P<0.05).The results of the Kaplan-Meier (K-M) curve showed that the overall survival (OS) of patients with high RRM2 expression in lung cancer was significantly lower than that of patients with low expression (P<0.001). The expression level of RRM2 in human bronchial epithelial cells (HBE1) was significantly lower than that in NSCLC cell lines CALU3 and H1299(P<0.05). Compared with the control group and si-NC group, the OD450 values, apoptosis rates and proliferation/migration abilities in the si-RRM2-1 group decreased after 48 and 72 hours of culture (P<0.05). Conversely, the pc-RRM2-2 group exhibited increased OD450 values and proliferation/migration capacities versus control and pc-NC groups (P<0.05). Conclusion RRM2 silencing can inhibit the overexpression efficiency, proliferation, migration and invasion of NSCLC cells. Conversely, RRM2 overexpression can enhance the overexpression efficiency, proliferation activity, migration and invasion ability of NSCLC cells.

Objective To compare the safety and efficacy of argatroban and plasmin in the treatment of patients with acute ischemic stroke (AIS). Methods A total of 168 patients with AIS who were not eligible for intravenous thrombolysis within 48 hours of onset were randomly divided into argatroban group and plasmin group. The former was given intravenous infusion of Argatroban injection for 7 days on the basis of conventional antiplatelet therapy; the latter was given intravenous infusion of plasmin for 10 days on the basis of conventional antiplatelet therapy. The National Institutes of Health Stroke Scale (NHISS) score and the modified Rankin (mRS) score at 3-month follow-up were evaluated before treatment and 1 week after treatment to understand the changes of neurological function and prognosis. At the same time, cranial CT/MRI, blood biochemistry, coagulation and other examinations before and after treatment were performed to exclude adverse events (including symptomatic intracranial hemorrhage and other organ hemorrhage). Results The NHISS scores of the AIS patients in the argatroban group and plasmin group were significantly decreased after 1 week of treatment compared with those before treatment (P<0.01); the mRS was significantly decreased after 3 months of follow-up (P<0.05). The total clinical effective rate in the argatroban group was significantly higher than that in the plasmin group after 1 week of treatment (P<0.05). After 1 week treatment of the two groups, the re-examination of cranial CT and biochemical test indicators showed that there were no adverse events (P>0.05). Conclusion Early application of argatroban and plasmin in AIS patients can improve neurological deficits, and the two groups do not increase the occurrence of adverse events, and the clinical efficacy of argatroban is better.

Objective To observe the clinical effect of the treatment of traumatic lacrimal canalicular laceration with single canalicular artificial lacrimal duct and double canalicular artificial lacrimal duct. Methods Sixty patients (60 eyes) with lacrimal canalicular laceration who visited the Department of Ophthalmology, Affiliated Hospital of Chengde Medical University the first time from March 2017 to December 2021 were selected. According to different surgical methods, patients were divided into group A(27 patients )and group B(33 patients). Group A was treated with single canalicular placement of artificial lacrimal duct for canalicular laceration anastomosis, and group B was treated with double canalicular placement of artificial lacrimal duct for canalicular laceration anastomosis. After 6 months ,we compared the patency of lacrimal passage and complications between the two groups. Results The cure rate of group A was 48.15%(13/27) and that of group B was 81.82%(27/33). There was significant difference between the two groups (P<0.05). Conclusion The treatment of lower lacrimal canalicular laceration with double canaliculi implantation artificial lacrimal duct is effective, the reduction of lacrimal canaliculi is closer to the anatomical structure, and there are few complications. It is an ideal surgical method for the treatment of traumatic lower lacrimal canalicular laceration.

Objective To analyze the expression of miR-125b-5p in lung adenocarcinoma (LUAD), and predict its target genes and prognostic value by bioinformatics analysis. Methods Tissue and cell chips of cisplatin resistance-related LUAD in Gene Expression Omnibus (GEO) database were obtained, and differentially expressed miRNAs were screened by R software. The miRBase database was used to analyze conservativeness, and the dbDEMC3.0 database was used to evaluate the expression level. The target genes were predicted by TargetScan, miRDB and miRTarBase databases, and were analyzed for functional term enrichment with the DAVID database. Combined with the survival information of LUAD patients in the Cancer Genome Atlas (TCGA) database, prognostic analysis was performed. Quantitative Real-time PCR (qRT-PCR) detection technology was used to detect the expression level of miR-125b-5p in human normal bronchial epithelial cell line 16HBE, LUAD cell line A549, and cisplatin-resistance cell line A549/DDP. Results miR-125b-5p, closely related to cisplatin resistant of LUAD, was obtained by analysis of the chip data. The sequence of miR-125b-5p was highly conserved among species, and miR-125b-5p was significantly down-regulated in various tumors including LUAD. Fifty-four target genes of miR-125b-5p were predicted. The results of enrichment analysis showed that the target genes of miR-125b-5p were mainly involved in the regulation of gene expression, cellular macromolecule biosynthetic process, and mainly enriched on MicroRNAs in cancer, Protein processing in endoplasmic reticulum, and HIF-1 signaling pathway. Survival analysis indicated that miR-125b-5p was significantly associated with poor prognosis of patients with LUAD. qRT-PCR results showed that the expression level of miR-125b-5p in A549 was significantly lower than in 16HBE (P=0.008). Compared to parental cell, the expression level of miR-125b-5p was significantly down-regulated in A549/DDP cell (P=0.023). Conclusion miR-125b-5p was abnormally expressed in LUAD, and its target genes involved in multiple biological processes and signal pathways, which might be used as a new biomarker for prognosis in LUAD.

Objective To study the influence of ginkgo extract on diabetic nephropathy. Methods A total of 80 patients with diabetic nephropathy were randomly divided into two groups. Based on the routine treatment of diabetes, ginkgo extract was added to experimental group. After 12 weeks of treatment, creatinine (CRE), uric acid (UA), urea nitrogen (BUN) and 24h urinary protein (24h Pro) were measured. At the same time, C-reactive protein (CRP), D-dimer (DD), plasma fibrinogen (FIB) and serum homocysteine (HCY) were determined as the basis of efficacy evaluation. Results The values of CRE, 24h proteinuria, DD and CRP in the experimental group were lower than those in the control group, and the difference was statistically significant(P<0.05). Conclusion Ginkgo biloba leaf extract can protect kidney function and improve blood hypercoagulability, which is effective in the treatment of diabetic nephropathy and can be popularized.