Objective To investigate the expression of IFITM3 in gastric cancer and its effect on malignant biological behavior of gastric cancer cells. Methods The expression of IFITM3 in gastric cancer was analyzed by database and the correlation between IFITM3 and overall survival rate of gastric cancer patients was analyzed. Si-ifitm3 was transfected into GASTRIC cancer cells SGC-7901, and cell proliferation, apoptosis and migration were analyzed by CCK-8 assay, flow cytometry and wound healing assay. The phosphorylation of P38-MAPK and the expression of EMT-related proteins (e-cadherin, Snail and Vimentin) were detected by Western blot. Results IFITM3 was highly expressed in gastric cancer and negatively correlated with the overall survival rate of gastric cancer patients. Transfection of SI-IFITM3 in SGC-7901 cells could effectively knock down the expression of IFITM3. Knocking down IFITM3 can significantly inhibit cell proliferation and migration, induce cell apoptosis, and also reduce the phosphorylation of P38-MAPK and the expression of EMT-related proteins. Conclusion Knockdown IFITM3 can inhibit EMT of gastric cancer cells by reducing phosphorylation of P38-MAPK protein, thus slowing down cell proliferation and migration, and inducing cell apoptosis.

Objective: To study the expression and significance of migration induced protein-7 (MIG-7) and matrix metalloproteinase-2 (MMP-2) in colon cancer. Methods: Immunohistochemical staining was used to detect the expression of MIG-7 and MMP-2 in 70 cases of colon cancer tissues and corresponding adjacent normal tissues. The relationships between MIG-7, MMP-2 and the clinicopathological characteristics of colon cancer, and the correlations between MIG-7 and MMP-2 in colon cancer tissues were also analyzed. Results: The positive expression rate of MIG-7 and MMP-2 in 70 cases of colon cancer tissues were 61.43% and 70.00% respectively, which were all singnificantly higher than adjacent normal tissues (P˂0.05). In colon cancer, the expression of MIG-7 and MMP-2 were related to differentiation and lymph node metastasis (P˂0.05); but irrelevant to sex, age and tumor size (P>0.05). In colon cancer, the expression of MIG-7 was positively correlated with MMP-2 (r=0.506,P˂0.05). Conduidons: MIG-7 and MMP-2 may participate in the growth and metastasis of colon cancer together.

Objective: To comparatively analyze 4 different extraction parts (A.50% ethanol extract from hawthorn leaf,B.alcohol extract of hawthorn leaf after macroporous resin,C.ethyl acetate part of hawthorn leaf,D.n-butanol part of hawthorn leaf). of hawthorn leaves by establishing HPLC finger-print and detecting total flavonoids content. Methods: The fingerprint of hawthorn leaves was established by HPLC method using Diamonsil C₁₈ (4.6 mm×250 mm, 5 μm) chromatographic column, acetonitrile-0.1% acetic acid-tetrahydrofuran as mobile phase with gradient elution, detection wavelength 320nm, flow rate of 0.9ml/min, and the column temperature was 30℃. The total flavonoids content was detected by ultraviolet spectrophotometry. Results: The common peaks of 4 extraction parts of hawthorn leaves in types and numbers had differences; The differences between extract C and the other three extracts was the largest. The total flavonoids content in extract B was the highest. Conclusions: This study can lay foundation for further development and utilization of hawthorn leaves extracts, and also can provide references for studying pharmacodynamic differences of different extraction parts of hawthorn leaves extracts.

Objective: To comparatively analyze the hawthorn leaves in south and north China by establishing HPLC fingerprints based on principal component analysis (PCA). Methods: HPLC was used to analyze 16 batches of hawthorn leaves from south (6 batches) and north (10 batches) China, and established HPLC fingerprints based on PCA. Results: 9 batches from north China and 6 batches from south China were selected to establish the UPLC fingerprints of hawthorn leaves based on PCA. The HPLC fingerprints of hawthorn leaves from north China had 16 common peaks, and the similarity was 0.808-0.972; the HPLC fingerprints of hawthorn leaves from south China had 23 common peaks, and the similarity was 0.923-0.979. Conclusions: The HPLC fingerprints of hawthorn leaves based on PCA in this study can objectively evaluate the differences of hawthorn leaves in south and north China, so it can provide references for clinical application of hawthorn leaves.

Objective: To establish hawthorn leaf fingerprint common patterns by using UPLC combined similarity calculation and clustering analysis, and provide reference for the quality evaluation of hawthorn leaves. Methods: The UPLC was used with the condition that the column was Waters CORTECS UPLC C₁₈ column (3.0mm ×100mm,1.6μm); the mobile phase was eluted with gradient by acetonitrilee-0.1% formic acid; the flow rate was 0.2ml/min; the detection wavelength was 320nm; the column temperature was 30℃; the sample volume was 3μl. The UPLC fingerprint of 14 batches of hawthorn leaves was established by evaluation system of traditional Chinese medicine chromatographic fingerprint similarity (2004A version), and the similarity calculation and cluster analysis were carried out. Results: The 14 batches of hawthorn leaves were divided into Ⅰ, Ⅱ group by cluster analysis; and the hawthorn leaf fingerprint common pattern was established based on Ⅰ group. Conclusions: The method established in this study can provide reference for quality control of hawthorn leaves.