Objective To investigate the effect of knocking down LncRNA TCONS-00022381 on apoptosis of human gastric cancer cells MKN45. Methods Culturing Human gastric cancer MKN45 cells and dividing them into three groups. Cells in each group were transfected using the Liposome transfection method, the siRNA targrting LncRNA TCONS-00022381 was transfected into the si-TCONS group, the negative siRNA was transfected into the NC group, and the Blank group was cultured in routine. The cell viability of each group was measured after 24h, 48h, and 72h of transfection using the CCK8 assay TCONS knockdown efficiency was detected by RTqPCR after 24h of transfection. The expression of Bcl-2 and Bax was detected by western blotting after 48h hours of transfection. The apoptotic rate of cells was detected by flow cytometry after 48h of transfection. Results The siRNA targeting LncRNA TCONS-00022381 significantly inhibited its expression in MKN45 cells (P<0.001). Compared with the Blank group and the NC group, the viability of MKN45 cell in the si-TCONS group decreased significantly (P<0.001). The expression of Bcl-2 was significantly down-regulated (P<0.01). The expression of Bax was significantly up-regulated (P<0.01). The rate of cell apoptosis increased significantly (P<0.05). Conclusion Knock down of LncRNA TCONS-00022381 may promote the apoptosis of MKN45 cells by regulating the expression of Bcl-2 and Bax.

Objective To analyze the correlation between serum levels of galectin-13 (Gal-3), serum amyloid A protein(SAA), interleukin-33 (IL-33) and the severity of allergic asthma in children. Methods A total of 102 children with allergic asthma in Xuchang People's Hospital from September 2021 to June 2023 were selected as the study group, and 102 healthy children in the same period and at the same age were selected as the control group. The serum levels of Gal-3, SAA, IL-33, pulmonary function [forced vital capacity (FVC), peak expiratory flow (PEF)], and exhaled nitric oxide (FeNO) were compared between the two groups. The correlations of serum Gal-3, SAA, IL-33 levels with pulmonary function, FeNO, and disease severity were analyzed. Results The levels of serum Gal-3, SAA and IL-33 in the study group were higher than those in the control group (P < 0.05). PEF and FVC in the study group were lower than those in the control group, and FeNO was higher than that in the control group (P < 0.05). The serum levels of Gal-3, SAA, and IL-33 in mild children were lower than those in moderate and severe children (P < 0.05). PEF and FVC in the mild group > moderate > severe, FeNO mild < moderate < severe (P < 0.05). Serum levels of Gal-3, SAA and IL-33 were negatively correlated with PEF and FVC, and positively correlated with FeNO and disease severity (P < 0.05). Conclusion The levels of serum Gal-3, SAA and IL-33 in children with allergic asthma are up-regulated, which are closely related to lung function, airway inflammation and disease severity, and have important clinical significance in judging the severity of the disease.

Objective To investigate the effects of low-expression of annexin A7 (ANXA7) on the proliferation and apoptosis of human gastric cancer MGC-803 cells. Methods Human gastric cancer MGC-803 cells were divided into ANXA7 low-expression group (si-ANXA7), negative control group (si-con) . Si-RNA targeting ANXA7 and negative control RNA were transfected into ANXA7 low-expression group and negative control group respectively. The expression of ANXA7 was detected by Western blot; MTT assay was used to detect the proliferation of cells in each group; The apoptosis rate in each group was detected by PI; The expressions of PCNA and Cleaved casepase-3 were detected in each group by Western blot. Results Compared with the negative control group, the proliferation ability of MGC-803 cells in the low-expression group of ANXA7 decreased significantly, and the expression of proliferation related proteins PCNA decreased significantly (P<0.05); The expression of cleaved casepase-3 increased significantly (P<0.05). Conclusion The low-expression of ANXA7 may inhibitthe proliferation and promote the apoptosis of human gastric cancer MGC-803 cells.

Objective To investigate the effects of low expression of Annexin A7 (ANXA7) on migration and invasion of human gastric cancer cells MGC-803. Methods Human gastric cancer MGC-803 cells were cultured and divided into 3 groups. Liposome transfection was used to transfect the targeted ANXA7 siRNA into the interference group cells, negative control siRNA was transfected into the negative control group, and the blank control group did not deal with any treatment. Cells from each group were tested for ANXA7 interference efficiency by Western blotting forty eight hours after transfection. Then the migration and invasion abilities of MGC-803 cells were tested by Wound healing assay and Transwell assay.The expression levels of E-cadherin and MMP-9 were determined by Western blotting. Results siRNA targeting ANXA7 significantly inhibited its expression in MGC-803 cells（P<0.05）; The migration and invasion ability of the interference group were significantly lower than that of the negative group and the blank group（P<0.05）; The expression of E-cadherin was significantly up-regulated（P<0.05）, mMP-9 expression was significantly down-regulated（P<0.05）. Conclusion The low expression of ANXA7 may inhibit the migration and invasion of MGC-803 cells by regulating the expression of E-cadherin and MMP-9.

Objective To investigate the effects of annexinA7 (ANXA7) overexpression on the proliferation and apoptosis of human gastric cancer MGC-803 cells. Methods Human gastric cancer MGC-803 cells were divided into ANXA7 overexpression group and negative control group. PcDNA3.1-ANXA7 recombinant plasmid and empty plasmid were transfected into ANXA7 overexpression group and negative control group respectively by liposome transfection. The overexpression of ANXA7 was detected by Western blot. CCK-8 assay was used to detect the proliferation ability of cells in each group. Cell apoptosis rate was detected by flow cytometry, and the expressions of PCNA, CyclinD1, Casepase-3 and Cleaved Casepase-3 were detected by Western blot. Results Compared with the negative control group, the proliferation ability of MGC-803 cells in the ANXA7 overexpression group was significantly enhanced, and the expression of PCNA and CyclinD1 were both increased (P<0.05). Apoptosis rate and Cleaved casepase-3 expression were decreased (P<0.05). The expression of Casepase-3 was not statistically significant (P>0.05). Conclusion The overexpression of ANXA7 can promote the proliferation and inhibit the apoptosis of human gastric cancer MGC-803 cells.

Objective To detect the expression of LncRNA TCONS_00022381 in gastric cancer and its effect on proliferation of SGC-7901 cells. Methods The expression of[] LncRNA TCONS_00022381 was determined by real-time quantitative PCR in gastric cancer samples and cell lines. The gastric cancer SGC-7901 cells were grouped into interference group, negative control group and blank group. The gastric cancer cells transfected with siRNA targeted to LncRNA TCONS_00022381 was designated as siRNA interference group, while named those transfected with scrambled siRNA as negative control group and the cells without any treatment as blank group. After transfection of 48 hours, real-time quantitative PCR was used to detect the suppression of LncRNA TCONS_00022381. The proliferation of each group was detected by MTT assay. The expression of proliferating nuclear antigen (PCNA) in each group was detected by Western blot. Results LncRNA TCONS_00022381 was up-regulated in both gastric cancer tissue samples and cell lines (P<0.05). siRNA transfection could significantly reduce the expression of LncRNA TCONS_00022381 in SGC-7901 cells(P<0.05). Compared with the negative and blank groups, the MTT assay showed that the proliferation activity and the PCNA expression were significantly down-regulated (P<0.05). Conclusion LncRNA TCONS_00022381 is highly expressed in gastric cancer and play a role in promoting the proliferation of gastric cancer cells.