Objective To explore the molecular mechanism of Smurf2's effect on hyperglycemia-induced mesangial cell proliferation and fibrosis by targeting EYA2. Methods Mouse mesangial cells (GMCs) were cultured, and sh-Smurf2 and sh-NC vectors were transfected into GMCs induced by high glucose. Cell proliferation in blank control group (NG), high glucose treatment group (HG), high glucose + sh-NC group (HG + sh-NC), and high glucose + sh-Smurf2 group (HG + sh-Smurf2) were detected by CCK-8 and EdU. The expressions of Fibronectin, Collagen I and α-SMA proteins were detected by Western blot. CO-IP assay was employed to detect the targeting interaction between Smurf2 and EYA2, and then sh-EYA2 and sh-NC vectors were used to transfect GMCs for backtracking experiments. Results Compared with NG group, cell proliferation and protein expression of Fibronectin, Collagen I and α-SMA in HG group were significantly increased. Compared with HG group, interference with Smurf2 inhibited cell proliferation and the expression of Fibronectin, Collagen I and α-SMA proteins. CO-IP results showed a targeted interaction between Smurf2 and EYA2. Compared with HG + sh-Smurf2 group, interference with EYA2 promoted cell proliferation and the expression of Fibronectin, Collagen I and α-SMA proteins. Conclusion Interference with Smurf2 can inhibit hyperproliferation and fibrosis of GMCs induced by high glucose by upregulating EYA2.

Objective The distribution and length of the tear area and the degree of the contracture of the broken end of the rotator cuff tear image were measured and compared with the tear morphology seen during the operation. Methods From October 2019 to December 2021, a total of 38 patients who met the research criteria and underwent arthroscopic or open repair due to rotator cuff tear were collected. The distribution area, length, and degree of contracture of rotator cuff tear were measured by this method, and the general shape of the tear was described before surgery and compared with the intraoperative situation. The main comparison parameters included distribution area and length of preoperative MRI oblique sagittal and intraoperative tears, and degree of oblique coronal contracture. Paired sample rank sum test was used for statistical analysis. The preoperative MRI measurements and intraoperative measurements were compared for statistical differences. At the same time, the common area of tears was calculated, and the consistency of preoperative and intraoperative tear morphology was compared. Results A total of 38 patients (20 males and 18 females), ranging in age from 37 to 73 years, were enrolled. There was no significant difference in rotator cuff tear length and contracture degree measured by oblique coronal MRI before operation (P=0.427; P=1.000). There was no significant difference in the length of rotator cuff tear and the degree of contracture between the preoperative MRI and the actual incision measurement (P=0.341; P=1.000). There was no significant difference between preoperative MRI and actual intraoperative microscopic measurement (P=0.865; P =1.000). Statistical analysis of rotator cuff tear zones showed that there was no significant statistical difference between the origin and end areas of the tear shown by preoperative MRI and the actual intraoperative tear origin and end areas (P=0.180; P=0.411), and the distribution frequency of tear zone was high in zones 1~5. Conclusion Preoperative MRI measurement and description of tear partition can show the tear morphology relatively intuitively, and has good consistency with the tear morphology seen under microscope and incision. It is helpful to plan the distribution of anchor and suture method during operation. Shorten the learning curve of shoulder arthroscopy. To improve the consistency of interpretation of tear morphology between surgeons and radiologists.

Objective To compare the effects of DSA-guided Fogarty balloon catheter removal and incision exploration in the treatment of arterial crisis combined with thrombosis. Methods Analysis rstrospectively the clinical data of 35 cases who had vascular crisis after replantation of severed limbs and diagnosed with combined thrombosis by high-frequency ultrasound from September 2012 to May 2020 in the Affiliated Hospital of Chengde Medical University. According to the surgical method, the cases divided into minimally invasive group (fogarty balloon catheter thrombectomy under DSA guidance, 12 cases) and open group (incision and exploratory surgery, 23 cases). A comparative study of various indicators before, during and after the operation. Results There was no statistically significant difference in general information between the two groups at admission (P>0.05), and they were comparable; the time to vascular repatency, intraoperative operation time, and intraoperative blood loss in the minimally invasive group were significantly lower than those in the open group (P<0.05); the postoperative re-embolization rate and postoperative amputation rate of the minimally invasive group were significantly lower than those of the open group (P<0.05); there was no significant difference between the two groups in the rate of good limb function at 9 months postoperative follow-up (P>0.05); the hospitalization medical expenses open group was significantly lower than the minimally invasive group (P>0.05). Conclusion DSA-guided Fogarty balloon catheter removal for arterial crisis combined with thrombosis can avoid secondary damage to the repair area of the broken end, with low incidence of complications and definite early curative effect, but will increase medical costs.