Objective To investigate the effect of circular RNA hsa-circ-0001862 on the phenotype of tongue squamous cell carcinoma and its mechanism on the occurrence and development of tongue squamous cell carcinoma. Methods hsa-circ-0001862 plasmid was constructed, and the interaction relationship between hsa-circ-0001862 and miR-23a-3p was verified by double luciferase reporter gene and qRT-PCR. After targeted inhibition of hsa-circ-0001862, CCK8 assay and colony formation assay were used to detect the cell viability and colony formation ability of tongue squamous cell carcinoma cells. The migration and invasion ability of tongue squamous cell carcinoma cells were detected by scratch test and Transwell test. Apoptosis-related proteins were detected by Western blot to reflect the effect of hsa-circ-0001862 on the apoptosis of tongue squamous cell carcinoma cells. Results hsa-circ-0001862 and miR-23a-3p could interact, and their expression was negatively correlated in tongue squamous cell, carcinoma cells. In Tca-8113 cells, hsa-circ-0001862 inhibited cell proliferation, migration and invasion (P<0.01), and promoted cell apoptosis (P<0.01). Conclusion hsa-circ-0001862 interacts with miR-23a-3p, and hsa-circ-0001862 plays an inhibitory role in the development of tongue squamous cell carcinoma. hsa-circ-0001862 may be a new biomarker and target for the treatment of tongue squamous cell carcinoma.

Objective To explore the influence of IRF1 on invasion and migration of tongue squamous cell carcinoma cells. Methods The expression level of IRF1 in tongue squamous cell carcinoma was detected by real-time quantitative PCR and immunohistochemistry. Construction of plasmids for IRF1 overexpression and silencing. Tca8113 was tongue squamous carcinoma cell lines. The effect of over-expressing IRF1 or reducing IRF1 expression level on Tca8113 cell proliferation, invasion and migration was detected. Results The expression of IRF1 in tongue squamous cell carcinoma tissues decreased abnormally, and the results of real-time quantitative PCR and immunohistochemistry were significantly lower than those in para-cancer tissue. The IRF1 over-expression and silencing plasmid were successfully constructed. Growth curve experiments showed that over-expression of IRF1 promoted proliferation of Tca8113 cells, while knockdown of IRF1 inhibited proliferation of Tca8113 cells. The scratch test showed that over-expression of IRF1 promoted the migration of Tca8113 cells, and knockdown IRF1 inhibited the migration of Tca8113 cells. Transwell experiments showed that over-expression of IRF1 promoted the invasion of Tca8113 cells, while knockdown of IRF1 inhibited the invasion of Tca8113 cells. Conclusion IRF1 played a role as a tumor suppressor gene in the development of tongue squamous cell carcinoma. The expression level of IRF1 decreased in tongue squamous cell carcinoma.