Objective To study the value of allograft inflammatory factor 1(AIF-1), interleukin-6(IL-6) and vascular endothelial growth factor(VEGF) in the diagnosis and prognosis assessment of non-small cell lung cancer(NSCLC). Methods A total of 183 NSCLC patients and 120 healthy subjects were selected.and divided into NSCLC group and control group The expression of serum AIF-1, IL-6 and VEGF were compared between the two groups. The relationship between them and tumor markers such as CEA, SCCA, CYFRA21-1 and clinicopathological features of NSCLC was analyzed. ROC curve was employed to analyze the diagnostic value of the three factors in NSCLC. The relationship between serum AIF-1, IL-6, VEGF and survival was analyzed. Results The serum levels of AIF-1, IL-6 and VEGF in NSCLC patients were higher than those in healthy controls (P<0.05), was positively correlated with CEA, SCCA and CYFRA21-1 (P<0.05). The three factors were related to lymph node metastasis, distant metastasis, tumor differentiation and TNM stage (P<0.05), IL-6 was also associated with cancer tissue size(P<0.05). ROC curve analysis showed that the sensitivity, specificity, area under the curve(AUC) and 95% CI of the three combined detection were 0.88, 0.78, 0.88, 0.84-0.92, respectively, which were better than those of single index. Survival analysis showed that the overall survival rate of NSCLC patients with high expression of AIF-1 and IL-6 was significantly lower than that with low expression of AIF-1 and IL-6 (P<0.05). Conclusion AIF-1, IL-6 and VEGF are involved in the occurrence and development of NSCLC, which are helpful for the clinical diagnosis and prognosis evaluation of NSCLC.

Objective To investigate the correlation between serum laten transforming growth factor binding protein 2 (LTBP2), type Ⅳ collagen α3 (COL4A3), carbohydrate antigen 125 (CA125) levels and clinicopathological features in patients with non-small cell lung cancer (NSCLC) and their diagnostic value. Methods A total of 97 patients with NSCLC admitted to Zhoukou Central Hospital from May 2021 to May 2023 were included in the observation group, and 97 patients with benign lung lesions were included in the control group. The serum levels of LTBP2 and COL4A3 were measured by double antibody enzyme-linked immunosorbent assay, and the serum levels of CA125 were measured by double antibody sandwich method. The serum levels of the two groups were compared, and the correlation between the levels of each index and pathological characteristics and the diagnostic value of combined detection for NSCLC were analyzed. The correlation between each index level and pathological characteristics was analyzed, as well as the diagnostic value of combined detection in NSCLC. Results Serum LTBP2, COL4A3 and CA125 levels in observation group were higher than those in control group (P<0.05). Serum levels of LTBP2, COL4A3 and CA125 in patients with different TNM stages, depth of invasion and lymph node metastasis were compared: Ⅲ~Ⅳ>Ⅰ~Ⅱ, T3+T4 > T1+T2, with lymph node metastasis > without lymph node metastasis (P<0.05). Serum levels of LTBP2, COL4A3 and CA125 were positively correlated with TNM stage, invasion depth and lymph node metastasis in NSCLC patients. The AUC of all indexes in the combined diagnosis of NSCLC was 0.889, the best sensitivity and specificity were 90.72% and 84.54%, respectively, and the Yoden index was 0.753. Conclusion Serum LTBP2, COL4A3 and CA125 levels are positively correlated with clinicopathological features of patients with NSCLC. Combined detection has certain diagnostic value for NSCLC, and can be used as auxiliary indicators for clinical diagnosis of NSCLC.

Objective To investigate the protective effect of catalpol on kidney and the expression of S100A8, TLR4 and NF-κB in diabetic rats. Methods Healthy male sprague-dawley rats were randomly divided into 3 groups: the normal control group, the diabetes model group, and the catalpol intervention group. The diabetic rats were induced by intraperitoneal injection with streptozotocin. The levels of fasting glucose, serum creatinine and urinary protein in each group were detected. The expression of S100A8, TLR4 and NF-κB in kidney were assessed by immunohistochemistry. The morphological changes of renal tissue were checked through microscopy. Results Compared with the model group, the levels of fasting blood glucose, serum creatinine and urinary protein in catalpol group were significantly decreased, and the expression levels of S100A8, TLR4 and NF-κB in renal cortex were dramatically decreased. Conclusion Catalpol has a protective effect on STZ induced diabetic rats, and its mechanism may be related to the decrease of fasting blood glucose level and the down regulation of S100A8, TLR4 and NF-κB protein expression in renal tissue.

Objective: To analyze the results of enzyme-linked immunosorbent assay (ELISA) and chemiluminescence immunoassay (CLIA) in detection of hepatitis B surface antigen (HBsAg) and Treponema pallidum (TP) antibody. Methods: ELISA and CLIA were used to detect HBsAg and TP antibody of 3060 blood samples. HBsAg negative samples were then detected by nucleic acid test (NAT). All the mismatching results of HBsAg and anti-TP detected by ELISA and CLIA were confirmed in National Center for Clinical Laboratories (NCCL). Results: HBsAg tests results: the sensitivity of ELISA and CLIA were all 90.00%, the specificity was 99.87% and 100.00% respectively. 4 cases ELISA positive CLIA negative samples were confirmed negative at last, but 1 positive sample of NAT was confirmed reactive. TP antibody tests results: the sensitivity of ELISA and CLIA was 40.00% and 90.00% respectively, the specificity was 99.84% and 99.97% respectively. 12 ELISA reactive CLIA negative samples were confirmed negative in 11 cases and reactive in 1 case; 8 CLIA positive ELISA negative samples, 6 samples were confirmed reactive while the other 2 were negative. Conclusions: CLIA has high sensitivity and specificity in detecting HBsAg and TP antibody of blood samples.

Objective: To investigate the effects of dihydromyricetin (DMY) on MicroRNA373 expression in choriocarcinoma JAR cells. Methods: JAR cells in logarithmic growth phase were treated with different concentrations of DMY (0μg/ml, 60μg/ml, 80μg/ml, 100μg/ml) for 24 hours, then the MicroRNA373 mRNA expression in JAR cells were detected by real time fluorescence quantitative PCR. Results: The MicroRNA373 expression of JAR cells in 3 DMY groups was all obviously higher than blank control group. Moreover, the MicroRNA373 expression gradually increased with increasing of DMY concentration (P<0.05). Conclusions: DMY can increase the MicroRNA373 expression in choriocarcinoma JAR cells in concentration dependent manner.