Objective This study utilizes network pharmacology and molecular docking techniques to explore the role of colchicine in regulating ferroptosis for the treatment of neurological injury. Methods Colchicine targets were obtained from the PubChem and Swiss Target Prediction databases, while targets related to neurological injury were retrieved from the GeneCards, OMIM, and DisGeNET databases. Ferroptosis targets were sourced from the FerrDb database, and the Venny 2.1.0 tool was accustomed to identify the intersection of these three sets. A protein-protein interaction (PPI) network was constructed based on the STRING database, and GO and KEGG pathway enrichment analyses were conducted using Metascape. Ultimately, molecular docking analyses of colchicine with the top ten targets were performed using Autodock Vina. Results There were 401 targets for colchicine, 3 367 for neurological injury, and 1 543 for ferroptosis, with 57 intersection targets. The PPI network identified TP53, IL6, EGFR, and others as major targets. KEGG analysis indicated that colchicine regulated ferroptosis through the Wnt signaling pathway to treat neurological injury. Molecular docking results showed that colchicine bound well to multiple targets. Conclusion Colchicine may regulate related genes through the Wnt signaling pathway to treat neurological injury.

Objective To investigate the effects of annexinA7 (ANXA7) overexpression on the proliferation and apoptosis of human gastric cancer MGC-803 cells. Methods Human gastric cancer MGC-803 cells were divided into ANXA7 overexpression group and negative control group. PcDNA3.1-ANXA7 recombinant plasmid and empty plasmid were transfected into ANXA7 overexpression group and negative control group respectively by liposome transfection. The overexpression of ANXA7 was detected by Western blot. CCK-8 assay was used to detect the proliferation ability of cells in each group. Cell apoptosis rate was detected by flow cytometry, and the expressions of PCNA, CyclinD1, Casepase-3 and Cleaved Casepase-3 were detected by Western blot. Results Compared with the negative control group, the proliferation ability of MGC-803 cells in the ANXA7 overexpression group was significantly enhanced, and the expression of PCNA and CyclinD1 were both increased (P<0.05). Apoptosis rate and Cleaved casepase-3 expression were decreased (P<0.05). The expression of Casepase-3 was not statistically significant (P>0.05). Conclusion The overexpression of ANXA7 can promote the proliferation and inhibit the apoptosis of human gastric cancer MGC-803 cells.

Objective To explore the feature of BMSC exosomeson sensory conduction function recovery post dorsal column injury. Methods BMSC exosomes were extracted from Wistar rats. 36 rats were randomly divided into sham, PBS control, and exosomes groups.The exosomes group was injected with exosomes via the tail vein, and the PBS control group was injected with the same amount of PBS. Tape removal test and somatosensory evoked potential were used to evaluate sensory conduction function. The spinal cord dorsal column was observed by immunofluorescence staining, and Western Blot was used to detect STAT3 and GAP43 protein expression. Results Immunofluorescence staining showed that the extracted BMSC could express CD29 and CD90. Western Blot showed that HSP70 and Alix were highly expressed in the extracted exosomes. Compared with the PBS control group, the exosomes group had a larger NF-200 staining area, and improved the waveform of somatosensory evoked potential and latency in tape removal test. The expression of STAT3 and GAP43 protein was significantly increased in exosomes group. Conclusion BMSC exosomes can promote the recovery of sensory conduction function of rats with spinal cord dorsal column lesion through STAT3/GAP43 pathway.

Objective: To explore the effects of bone marrow mesenchymal stem cell (BMSC) exosomes on STAT3/GAP43 pathway and axonal growth of primary sensory neurons. Methods: BMSC of Wistar rats was extracted and the cell morphology was observed and identified by light microscope. Hypercentrifugation was used to extract BMSCs exosomes and identified the exosomes. Primary sensory neurons (rat dorsal root ganglion neurons) were treated with BMSC exosomes, immunofluorescence staining was used to detect axon growth of primary sensory neurons, and Western Blotting to detect STAT3 and GAP43 protein expression in primary sensory neurons. Results: Immunofluorescence staining showed that the extracted cells expressed CD29 and CD90 simultaneously, which proved the extracted cells were BMSC. Western Blotting showed the extracted exosomes expressed HSP70 and Alix. Compared with the control group, the length of neuronal axon in BMSC exosomes group increased significantly, the expression of STAT3 and GAP43 protein also significantly increased in BMSC exosomes group (P<0.05). Conclusions: BMSC exosomes can promote axonal growth of primary sensory neurons by regulating STAT3/GAP43 pathway.