Objective To explore the influence of IRF1 on invasion and migration of tongue squamous cell carcinoma cells. Methods The expression level of IRF1 in tongue squamous cell carcinoma was detected by real-time quantitative PCR and immunohistochemistry. Construction of plasmids for IRF1 overexpression and silencing. Tca8113 was tongue squamous carcinoma cell lines. The effect of over-expressing IRF1 or reducing IRF1 expression level on Tca8113 cell proliferation, invasion and migration was detected. Results The expression of IRF1 in tongue squamous cell carcinoma tissues decreased abnormally, and the results of real-time quantitative PCR and immunohistochemistry were significantly lower than those in para-cancer tissue. The IRF1 over-expression and silencing plasmid were successfully constructed. Growth curve experiments showed that over-expression of IRF1 promoted proliferation of Tca8113 cells, while knockdown of IRF1 inhibited proliferation of Tca8113 cells. The scratch test showed that over-expression of IRF1 promoted the migration of Tca8113 cells, and knockdown IRF1 inhibited the migration of Tca8113 cells. Transwell experiments showed that over-expression of IRF1 promoted the invasion of Tca8113 cells, while knockdown of IRF1 inhibited the invasion of Tca8113 cells. Conclusion IRF1 played a role as a tumor suppressor gene in the development of tongue squamous cell carcinoma. The expression level of IRF1 decreased in tongue squamous cell carcinoma.

Objective: To investigate the expression and significance of c-met, vascular endothelial growth factor C (VEGF-C) and lymphatic microvessel density (LMVD) in esophageal squamous carcinoma. Methods: Immunohistochemical staining was used to detect the expression of c-met and VEGF-C, and LMVD was calculated by D2-40 in 25 cases of normal esophageal mucosa and 60 cases of esophageal squamous carcinoma; and the relationships between c-met, VEGF-C, LMVD and clinicopathologic features of esophageal squamous carcinoma were also analyzed. Results: The expression of c-met, VEGF-C and LMVD in esophageal squamous carcinoma were significantly higher than normal esophageal mucosa (P<0.05). c-met and VEGF-C expression in esophageal squamous carcinoma were related to lymph node metastasis and TNM stage (P<0.05); while LMVD was related to infiltration depth, lymph node metastasis and TNM stage (P<0.05). Conclusions: c-met, VEGF-C and lymphoangiogenesis may play important role in development, invasion and metastasis of esophageal squamous carcinoma.