Objective To establish a visual loop-mediated isothermal amplification detection method for rapid detection of Staphylococcus epidermidis. Methods A loop-mediated isothermal amplification system was established by selecting specific primers for SesB gene of Staphylococcus epidermidis. The visualization method of the reaction, the concentration of MgSO₄, betaine in the system and the reaction temperature, time were optimized. The sensitivity and specificity of the optimized LAMP reaction and PCR reaction were compared. Results The optimal MgSO₄ concentration in the loop-mediated isothermal amplification reaction system was 4mM. The optimal betaine concentration was 0.4M. The optimal reaction temperature and time were 63℃ for 60 min. The most suitable visualization method was calcein chromogenic method, and the visualization results were basically consistent with the results of agarose gel electrophoresis. Both LAMP and PCR reactions had good specificity. The sensitivity of LAMP reaction was 10 copies/μL, and the sensitivity of PCR reaction was 100 copies/μL. Conclusion LAMP assay is superior to PCR in the detection of Staphylococcus epidermidis infection. It is more suitable for grass-roots and remote areas due to its simple operation and short reaction time.

Objective: To investigate the inhibiting effects of picropodophyllin on human breast cancer MCF-7 cell proliferation. Methods: Human breast cancer MCF-7 cells were cultured in vitro, and MTT method was used to detect the inhibitory effects of different concentrations of picropodophyllin (0.1μmol/L, 0.2μmol/L, 0.4μmol/L, 0.8μmol/L, 1.6μmol/L,3.2μmol/L, 6.4μmol/L) on proliferation of MCF-7 cells for 24h, 48h and 72h. At the same time, the structural changes of MCF-7 cells were observed under inverted microscope at different concentrations of picropodophyllin for 48h. Results: The concentration range was 0.1μmol/L~ 6.4μmol/L; and the growth inhibiting rate of MCF-7 cells increased gradually with the increasing of picropodophyllin concentration and action time (P<0.05). The IC₅₀ of MCF-7 cells treated with picropodophyllin for 24h, 48h and 72h were 7.32μmol/L, 0.720μmol/L and 0.318μmol/L respectively. Under inverted microscope, the MCF-7 cells contracted with unclear structure and the refractivity reduced after treated with picropodophyllin for 48h. Conclusions: Picropodophyllin has significant inhibiting effects on human breast cancer MCF-7 cells.

Objective: To compare the clinical effects and safety pemetrexed combined with platinum and other platinum duplex regimens for treating non-drive gene positive advanced lung adenocarcinoma. Methods: 120 cases of non-drive gene positive advanced lung adenocarcinoma patients were divided into experimental group (n=55) and control group (n=65) according to treatment. The patients in experimetal group were treated with pemetrexed combined with platinum regimens, while the patients in control group were treated with TP(taxol+cisplatin) / DP(docetaxel+cisplatin) / GP(gemcitabine+cisplatin) chemotherapy regimens. The curative effects and safety of patients in 2 groups were evaluated after 4 treatment cycles. Results: The overall response rate (ORR), disease control rate (DCR), overall survival (OS), 1 year survival rate of patients in experimental group were obviously higher than control group (P<0.05). The incidence rate of thrombocytopenia, leukopenia and anemia of patients in experimental group were obviously lower than control group (P<0.05). Conclusions: Compared with TP/DP/GP regimen, treating non-drive gene positive advanced lung adenocarcinoma with PC regimen can get more significant effects and less adverse reactions, so it is worthy to be popularized in clinic.

Objective: To explore the expression and clinical significance of insulin-like growth factor 1 (IGF-1) in human breast cancer. Methods: MaxVision immunohistochemical staining and RT-PCR were used to detect the IGF-1 protein and mRNA expression in 80 cases of breast cancer, 60 cases of breast fibroadenoma and 50 cases of normal breast tissue adjacent to carcinoma; And the relationships between IGF-1 expression and clinicopathologic features of breast cancer were also analyzed. Results: The IGF-1 protein and mRNA expression in breast cancer were obviously higher than breast fibroadenoma and normal breast tissue adjacent to carcinoma (P<0.05). The expression of IGF-1 in breast cancer was not related to age (P>0.05), but was related to tumor size, lymph node metastasis and histological differentiation (P<0.05). Conclusions: High expression of IGF-1 may play important role in development of breast cancer.

Objective: To investigate the expression and clinical significance of insulin-like growth factor binding protein-3 (IGFBP-3) and matrix metalloproteinases-14 (MMP-14) in colorectal cancer (CRC). Methods: Immunohistochemical staining was used to detect the expression of IGFBP-3 and MMP-14 in 70 cases of CRC, 30 cases of colorectal adenoma (CRA) and 20 cases of normal colorectal mucosa (NM). The relationships between IGFBP-3, MMP-14 and clinical pathological features of CRC, as well as the correlations between IGFBP-3 and MMP-14 in CRC were analyzed. Results: In CRC, the MMP-14 protein expression was obviously higher than CRA and NM; the MMP-14 expression was concerned with depth of invasion, lymph node metastasis and Dukes stage (P<0.01, P<0.05). In CRC, the IGFBP-3 protein expression was obviously lower than CRA and NM; the IGFBP-3 expression was concerned with lymph node metastasis and Dukes stage (P<0.05). In CRC, the expression of MMP-14 was negatively correlated with the expression of IGFBP-3 (r=-0.460, P<0.05). Conclusions: IGFBP-3 and MMP-14 may have synergistic effects and participate in the process of development, invasion and metastasis of CRC jointly.