Objective To analyze the effect of eperisone hydrochloride combined with glucosamine hydrochloride on knee osteoarthritis. Methods A total of 99 patients with knee osteoarthritis admitted to Nanyang Nanshi Hospital from June 2020 to June 2022 were selected as the research objects and divided into control group (n=49) and study group (n=50). Patients in the control group were treated with glucosamine hydrochloride, while patients in the study group were treated with glucosamine hydrochloride and eperisone hydrochloride combined treatment. The indexes of oxidative stress and inflammatory factors were detected by ELISA, the knee joint function was evaluated by WOMAC score, and the joint ROM was used to evaluate the degree of joint mobility, and the total effective rate was observed. Results Compared with before treatment, the scores of nitric oxide (NO), malondialdehyde (MDA), TNF-α, MMP-3, interleukin-6 (IL-6), difficulty in daily activities, stiffness and pain in the two groups were decreased after treatment, and those in the study group were lower than the control group (P<0.05). Compared with before treatment, the levels of superoxide dismutase (SOD), active knee flexure ROM, and Lysholm score of the two groups were increased after treatment, and the study group was higher than the control group (P<0.05). Compared with the control group, the total effective rate of the study group was higher (P<0.05). Conclusion The combined treatment of eperisone hydrochloride and glucosamine hydrochloride can reduce TNF-α and MMP-3 in patients with knee osteoarthritis, improve the oxidative stress state of patients, and the clinical effect is good.

Objective To investigate the protective effect of catalpol on kidney and the expression of S100A8, TLR4 and NF-κB in diabetic rats. Methods Healthy male sprague-dawley rats were randomly divided into 3 groups: the normal control group, the diabetes model group, and the catalpol intervention group. The diabetic rats were induced by intraperitoneal injection with streptozotocin. The levels of fasting glucose, serum creatinine and urinary protein in each group were detected. The expression of S100A8, TLR4 and NF-κB in kidney were assessed by immunohistochemistry. The morphological changes of renal tissue were checked through microscopy. Results Compared with the model group, the levels of fasting blood glucose, serum creatinine and urinary protein in catalpol group were significantly decreased, and the expression levels of S100A8, TLR4 and NF-κB in renal cortex were dramatically decreased. Conclusion Catalpol has a protective effect on STZ induced diabetic rats, and its mechanism may be related to the decrease of fasting blood glucose level and the down regulation of S100A8, TLR4 and NF-κB protein expression in renal tissue.