Objective: To systematically evaluate the curative effects for treating endometrial carcinoma in elderly patients with laparoscopic and open surgery in China. Methods: The literatures were collected according to selection standard in WanFang database,CNKI, VIP and CBM; and the clinical trials (CCT) were statistically analyzed by RevMan 5.2. Results: 5 cases of CCT were analyzed in this study. Compared with open surgery, the laparoscopic surgery had shorter operation time, less intraoperative blood loss, early postoperative exhaust time, shorter postoperative indwelling catheter time and shorter hospital stays (P<0.05); But there was no statistical significance in number of lymph nodes clearance and surgical complications (P>0.05). Conclusions: Laparoscopic surgery is an ideal operation in the treatment of endometrial carcinoma in elderly patients in China, because it has the advantages of less-injury, quick recovery and without increasing complications.

Objective: To investigate the effects of inhibiting ARTN gene by targeted interference of ARTN gene on proliferation of endometrial cancer Ishikawa cells. Methods: Short hairpin RNA (pGPU6/GFP/Neo-ARTN shRNA) interference plasmid of ARTN gene was constructed using pGPU6/GFP/Neo as carrier, and transfected into human endometrial cancer Ishikawa cells by liposome method. The transfection efficiency was observe under fluorescence microscope; Western Blot was used to detected ARTN protein expression in Ishikawa cells after transfection; CCK method to detect the proliferative activity of Ishikawa cells. Results: The pGPU6/GFP/Neo-ARTN shRNA interference plasmid was successfully transfected into Ishikawa cells and the transfection efficiency was 80%. 48h after transfection, the ARTN protein expression of Ishikawa cells in interference group was obviously lower than negative control group and normal group (P<0.05). 48h, 72h and 96h after transfection, the proliferative activity of Ishikawa cells in interference group was obviously lower than negative control group and normal group (P<0.05). Conclusions: Targeted interference of ARTN gene can inhibit the proliferative activity of endometrial cancer Ishikawa cells.