Objective To detect the expression of LncRNA TCONS_00022381 in gastric cancer and its effect on proliferation of SGC-7901 cells. Methods The expression of[] LncRNA TCONS_00022381 was determined by real-time quantitative PCR in gastric cancer samples and cell lines. The gastric cancer SGC-7901 cells were grouped into interference group, negative control group and blank group. The gastric cancer cells transfected with siRNA targeted to LncRNA TCONS_00022381 was designated as siRNA interference group, while named those transfected with scrambled siRNA as negative control group and the cells without any treatment as blank group. After transfection of 48 hours, real-time quantitative PCR was used to detect the suppression of LncRNA TCONS_00022381. The proliferation of each group was detected by MTT assay. The expression of proliferating nuclear antigen (PCNA) in each group was detected by Western blot. Results LncRNA TCONS_00022381 was up-regulated in both gastric cancer tissue samples and cell lines (P<0.05). siRNA transfection could significantly reduce the expression of LncRNA TCONS_00022381 in SGC-7901 cells(P<0.05). Compared with the negative and blank groups, the MTT assay showed that the proliferation activity and the PCNA expression were significantly down-regulated (P<0.05). Conclusion LncRNA TCONS_00022381 is highly expressed in gastric cancer and play a role in promoting the proliferation of gastric cancer cells.

Objective: To investigate the effects of low expression of annexin A5 (ANXA5) on Galectin-3 expression in human gastric cancer cells. Methods: Human gastric cancer HGC-27 cells and MGC-803 cells were grouped in siRNA interference group, negative control group and blank control group. The cells in siRNA interference group and negative control group were respectively transfected with siRNA Oligo targeting ANXA5 and negative control siRNA Oligo. Western blotting and qRT-PCR were used to detect the ANXA5, Galectin-3 protein and mRNA expression in HGC-27 cells and MGC-803 cells. Results: After inhibiting ANXA5 expression, the Galectin-3 protein and mRNA expression in HGC-27 cells were all significantly decreased compared with HGC-27 cells in negative control group and blank control group (P<0.05). Compared with negative control group and blank control group, there had no significant changes of Galectin-3 expression in MGC-803 cells in siRNA interference group (P>0.05). Conclusions: ANXA5 has regulatory effects on Galectin-3 expression in human gastric cells, which may be related to differentiation degree of gastric cancer cells.