Objective To investigate the effect of LncRNA CUDR knock-down on invasion, proliferation and apoptosis of hepatocellular carcinoma cells. Methods CUDR shRNA lentivirus plasmid was used to transfect SMMC-7721 hepatoma cell line. The CUDR knockdown group, control group and empty plasmid group were set up respectively. The transfection efficiency of CUDR shRNA was detected by RT-PCR method, the invasive ability of the three groups was detected by Transwell, the ability of cell aggregation and proliferation was detected by tumor ball formation test, and the apoptosis rate of hepatocellular carcinoma cells was detected by flow cytometry. Results The results of RT-PCR detection showed that the expression of CUDR mRNA in the knockdown group was significantly lower than that in the control group and empty plasmid group after CUDR shRNA transfection（P<0.001）. The results of Transwell, tumor ball formation test and apoptosis assay showed that the invasion and aggregation abilities of hepatoma cells in the CUDR knockdown group were significantly lower than those in the control group and the empty plasmid group, and the apoptosis rate was increased in CUDR knockdown group(P<0.001). Conclusion Knocking down the expression of LncRNA CUDR can inhibit the invasion and proliferation of HCC and promote the apoptosis of hepatocellular carcinoma cells, LncRNA CUDR may be a potential therapeutic target for HCC.