Objective To investigate the effect of Erastin on the growth and metastasis of hepatocellular carcinoma Hepa1-6 cells in vitro. Methods Hepa1-6 cells were treated with Erastin at different concentrations. The proliferation and activity of Hepa1-6 cells were assessed using cell proliferation-cytotoxicity assays, specifically the MTT assay and the CCK-8 assay. The proliferative capacity of the cells was evaluated through the plate colony formation assay. Additionally, the invasive and migratory abilities of the cells were investigated using the Transwell assay, with the migratory ability further examined through the scratch assay. Results As the concentration of Erastin increased, the inhibition rate of proliferation in Hepa1-6 cells progressively rose, while the survival rate and proliferative capacity declined correspondingly. Additionally, there was a reduction in both the number of migrating and invading cells, leading to decreased cellular mobility. Conclusion Erastin significantly inhibits the proliferation of Hepa1-6 cells and also reduces their invasion and migration in a dose-dependent manner.

Objective The purpose of this study was to investigate the possible intervention targets and inhibitory effects of melatonin (MT) on Acute lung injury (ALI). Methods The main targets of MT and the disease targets of ALI were obtained through database screening. The common targets of MT and ALI were analyzed by Venn diagram. The network model of “drug-disease” was built by Cytoscape 3.8.2. Protein interactions were analyzed by STRING and PPI networks were constructed. At the same time, an animal model of ALI was established and MT treatment was performed. The changes of lung histopathology were observed. The pulmonary function levels of Ri-area, Re-area and Cldyn-area in each group were detected by tracheotomy and intubation. Results 196 MT targets, 3948 ALI targets and 118 drug-disease co-targets were obtained. EGFR, HIF1A and ERBB2 were the key core sites of MT targeting ALI disease. In the GO enrichment analysis, a total of 1690 biological processes, 90 cellular components and 176 molecular functions were involved. As for KEGG pathway enrichment screening, a total of 157 biological pathways related to this were found. The results of in vivo experiments showed that MT therapeutic intervention had obvious inhibitory effect on ALI. Conclusion MT may inhibit the onset and progression of ALI through multiple targets.

Objective To analyze the expression and clinical significance of S1PR2, ApoH and Let-7d in aortic dissection. Methods A total of 60 patients with thoracic and abdominal aortic dissection diagnosed by CTA in Nanyang Central Hospital from January 2021 to September 2023 were selected as the study group, and another 50 healthy subjects during the same period were selected as the control group. The results of digital subtraction angiography (DSA) were used as the gold standard. The patients in the study group were divided into mild group (n=21), moderate group (n=19) and severe group (n=20) according to the severity of the disease. The expressions of S1PR2, ApoH and Let-7d in the two groups were compared, the expressions of S1PR2, ApoH and Let-7d in different levels of the groups, the detection comparison between various detection methods and the combined detection of the three groups, and the ROC curve analysis of S1PR2, ApoH, Let-7d and the combined diagnostic value of the three groups, S1PR2, ApoH and Le Association of t-7d levels with aortic dissection disease. Results Compared with the control group, the expression of S1PR2 and Let-7d in the study group was lower and the expression of ApoH was higher (P<0.05). The expression of S1PR2 and Let-7d in severe group was lower than that in mild and moderate groups, and the expression of ApoH in severe group was higher than that in mild and moderate groups. In this study, 5 cases were positive for S1PR2, 6 cases were positive for ApoH and 4 cases were positive for Let-7d, all of which were lower than the number of positive cases for combined testing. The combined AUC value of the three components (0.743) was higher than that of S1PR2 (0.552), ApoH (0.343) and Let-7d (0.493). The combined sensitivity, specificity and accuracy were higher (P<0.05). Based on the correlation analysis of S1PR2, ApoH, Let-7d and aortic dissection disease, the correlation between S1PR2 and aortic dissection disease showed a negative correlation (r=-5.137, P=0.027), and the correlation between ApoH and aortic dissection disease showed a positive correlation (r=4.211, P=0.044) and Let-7d were negatively correlated with aortic dissection disease (r=-6.291, P=0.016). Conclusion There is a certain correlation between the expression levels of S1PR2, ApoH and Let-7d in aortic dissection diseases. The combination of these three indicators can provide a theoretical basis for guiding the treatment and prognosis evaluation of aortic dissection diseases.