过表达LC3基因对横纹肌肉瘤RD细胞生物学行为的影响

承德医学院学报 ›› 2026, Vol. 43 ›› Issue (2): 95-100.

过表达LC3基因对横纹肌肉瘤RD细胞生物学行为的影响

吴晶晶^1,2, 闫涵^1,2, 吴恬菲^1,2, 堵舒桐^1,2,3,*

1.大理大学病原生物学实验室,云南大理 671000;
2.大理大学人体寄生虫教研室,云南大理 671000;
3.云南省自然疫源性疾病防控技术重点实验室,云南大理 671000

收稿日期:2025-04-18 出版日期:2026-04-10 发布日期:2026-05-07
通讯作者: *堵舒桐（1988—）,女,博士,副教授,研究方向：病原生物学;E-mail：86475710@qq.com。
作者简介:吴晶晶（2002—）,女,硕士研究生在读,研究方向：病原生物学。
基金资助:
大理大学博士科研项目（KYBS2018009）; 云南省教育厅科学研究基金项目（2024J0833）; 云南省科技厅地方本科高校基础研究联合专项面上项目（202401BA070001-081）

Effect of Overexpression of LC3 Gene on the Biological Behavior of Rhabdomyosarcoma RD Cells

WU Jingjing^1,2, YAN Han^1,2, WU Tianfei^1,2, DU Shutong^1,2,3,*

1. Pathogenic Biology Laboratory, Dali University, Dali, Yunnan, 671000, China;
2. Department of Human Parasitology, Dali University, Dali, Yunnan, 671000, China;
3. Yunnan Provincial Key Laboratory for Prevention and Control Technology of Natural Epidemic Diseases, Dali,Yunnan, 671000, China

Received:2025-04-18 Online:2026-04-10 Published:2026-05-07

摘要/Abstract

摘要： 目的探究过表达LC3基因介导的自噬对横纹肌肉瘤（RMS）RD细胞生物学作用的影响。方法采用转染技术将RD细胞分为阴性对照组（转染空载质粒pcDNA3.1）和LC3实验组（转染LC3质粒）。通过RT-qPCR技术及Western Blot技术验证转染LC3基因在mRNA水平及蛋白质水平的表达变化;采用CCK-8实验分析RD细胞增殖情况;运用细胞划痕愈合实验及Transwell实验检测细胞迁移和侵袭能力;使用TUNEL实验评估细胞凋亡水平。结果与阴性对照组相比,LC3实验组在RD细胞中的mRNA表达量显著升高（P<0.001）;其过表达可抑制RD细胞的增殖、迁移、侵袭（均P<0.05）,并促进其细胞凋亡（P<0.001）。结论 LC3基因可抑制RD细胞增殖、迁移、侵袭能力并促进细胞凋亡,有望成为治疗RMS新的靶基因。

关键词: 过表达, LC3基因, 自噬, 横纹肌肉瘤, 生物学行为

Abstract: Objective This study explored the effect of autophagy mediated by overexpression of the LC3 gene on the biological functions of rhabdomyosarcoma RD cells. Methods Rhabdomyosarcoma RD cells were divided into a negative control group (transfected with empty vector pcDNA3.1) and an LC3 experimental group (transfected with LC3 plasmid) using transfection technology. The mRNA and protein expression levels of the transfected LC3 gene were detected using RT-qPCR and Western blot assays. Cell proliferation was analyzed by the CCK-8 assay, while cell migration and invasion abilities were evaluated using wound healing and Transwell assays. Cell apoptosis was assessed by the TUNEL assay. Results Compared with the negative control group, the mRNA expression level was significantly increased in rhabdomyosarcoma RD cells in the LC3 experimental group (P<0.001). In addition, LC3 overexpression inhibited the proliferation, migration, and invasion of RD cells (all P<0.05), and promoted their apoptosis (P<0.001). Conclusion The LC3 gene inhibits the proliferation, migration, and invasion ability of RD cells and promotes cell apoptosis, and it is expected to become a novel target gene for the treatment of rhabdomyosarcoma.

Key words: overexpression, LC3 gene, autophagy, rhabdomyosarcoma, biological behavior

中图分类号:

R738.7

吴晶晶, 闫涵, 吴恬菲, 堵舒桐. 过表达LC3基因对横纹肌肉瘤RD细胞生物学行为的影响[J]. 承德医学院学报, 2026, 43(2): 95-100.

WU Jingjing, YAN Han, WU Tianfei, DU Shutong. Effect of Overexpression of LC3 Gene on the Biological Behavior of Rhabdomyosarcoma RD Cells[J]. Journal of Chengde Medical University, 2026, 43(2): 95-100.

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https://tougao.cdmc.edu.cn/CN/Y2026/V43/I2/95

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