桑白皮总黄酮对大鼠急性肝损伤的保护作用

承德医学院学报 ›› 2026, Vol. 43 ›› Issue (3): 185-191.

桑白皮总黄酮对大鼠急性肝损伤的保护作用

李博, 张凤怡, 尹浩羽, 白梦璇, 赵娟^*, 王途^*

承德医学院基础医学院,河北承德 067000

收稿日期:2025-06-18 出版日期:2026-06-25 发布日期:2026-06-25
通讯作者: ^*王途（1993—）男,硕士,讲师,研究方向：肝损伤机制研究;E-mail：wangtu522@cdmc.edu.cn;赵娟（1981—）女,博士,教授,研究方向：肝损伤机制研究;E-mail：zhaojuan@cdmc.edu.cn。
作者简介:李博（2003—）,女,本科在读,研究方向：急性肝损伤的预防。
基金资助:
河北省自然科学基金中医药联合基金重点项目（H2023406017）; 河北省自然科学基金（H2020406011）; 河北省教育厅高校重点学科建设项目（冀教高〔2013〕4号）; 河北省高等学校科学研究青年基金（QN2024116）; 承德医学院基本科研业务费（KY202227）; 承德医学院学科建设经费

Protective effect of total flavonoids from Cortex Mori on acute liver injury in rats

LI Bo, ZHANG Fengyi, YIN Haoyu, BAI Mengxuan, ZHAO Juan^*, WANG Tu^*

Basic Medical College, Chengde Medical University, Chengde, Hebei, 067000, China

Received:2025-06-18 Online:2026-06-25 Published:2026-06-25

摘要/Abstract

摘要： 目的探讨桑白皮总黄酮（TFMB）通过拮抗UⅡ/UT系统、调控JAK2/STAT3信号通路防治急性肝损伤（ALI）的作用。方法将大鼠随机分为对照组、ALI模型组、MgIG组、Urantide组、TFMB组。MgIG组和Urantide组分别静脉注射MgIG和Urantide,剂量为10 mg·kg^-1,TFMB组分别灌胃1.8、3.6、7.2 g·kg^-1;对照组和ALI模型组给予等量生理盐水,各组治疗周期均为连续14 d。结果 ALI模型组大鼠的病理表现为肝脏明显水肿、严重脂肪变性、肝窦扩张,EIF4EBP1、p-p70s6蛋白表达增高,同时UⅡ/UT系统及JAK2/STAT3通路被激活,p-JAK2、p-STAT3主要表达于损伤部位。用TFMB进行预防性干预后,与ALI模型组相比,肝脏病理症状明显减轻,p-p70s6、EIF4EBP1表达明显降低,UⅡ/UT系统及JAK2/STAT3通路激活状态被抑制。此外,在H₂O₂诱导的大鼠肝细胞（BRL3A）细胞模型中,TFMB对JAK2/STAT3通路也有抑制作用,起到抑制增殖的作用。结论 TFMB可能通过拮抗UⅡ/UT系统、调控JAK2/STAT3信号通路对CCl₄诱导的大鼠急性肝损伤起到防治作用。

关键词: 急性肝损伤, 桑白皮总黄酮, UⅡ/UT系统, JAK2/STAT3信号通路

Abstract: Objective To investigate the effect of total flavonoids from mulberry bark (TFMB) on acute liver injury (ALI) by antagonizing UⅡ/UT system and regulating JAK2/STAT3 signaling pathway. Methods Rats were randomly divided into control group, ALI model group, MgIG group, Urantide group and TFMB group. MgIG and Urantide were intravenously injected at a dose of 10 mg . kg^-1 in MgIG group and Urantide group, respectively. TFMB groups was gavaged with 1.8, 3.6 and 7.2 g . kg^-1, respectively; the control group and ALI model group were given the same amount of normal saline by gavage, and the treatment cycle of each group was continuous for 14 days. Results The ALI model of rats was reproduced with CCl₄, and the pathological manifestations were obvious liver edema, severe steatosis, hepatic Sinus Dilation, and the expression of EIF4EBP1 and p-p70s6 protein increased. At the same time, UⅡ/UT system and JAK2/STAT3 pathway were activated, and p-JAK2 and p-STAT3 were mainly expressed in the injured site. After preventive intervention with tfmb, compared with ALI model group, the pathological symptoms of liver were significantly reduced, the expression of p-P70s6 and EIF4EBP1 were significantly reduced, and the activation of UⅡ/UT system and JAK2/STAT3 pathway were inhibited. In addition, in the H₂O₂ induced rat hepatocyte (BRL3A) cell model, TFMB also inhibited JAK2/STAT3 pathway and proliferation. Conclusion TFMB may play a preventive and therapeutic role in CCl₄ induced acute liver injury in rats by antagonizing UⅡ/UT system and regulating JAK2/STAT3 signaling pathway.

Key words: acute liver injury, total flavonoids from mulberry bark, UⅡ/UT system, JAK2/STAT3 signaling pathway

中图分类号:

R285.5

李博, 张凤怡, 尹浩羽, 白梦璇, 赵娟, 王途. 桑白皮总黄酮对大鼠急性肝损伤的保护作用[J]. 承德医学院学报, 2026, 43(3): 185-191.

LI Bo, ZHANG Fengyi, YIN Haoyu, BAI Mengxuan, ZHAO Juan, WANG Tu. Protective effect of total flavonoids from Cortex Mori on acute liver injury in rats[J]. Journal of Chengde Medical University, 2026, 43(3): 185-191.

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https://tougao.cdmc.edu.cn/CN/Y2026/V43/I3/185

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